The Garcia-Marcos Lab has recently published the development and implementation of a suite of G protein biosensors broadly applicable to detect GPCR activity in scalable assay formats and in physiologically relevant systems like primary cells. By directly measuring endogenous GPCR activity, these biosensors, named ONE-GO, reveal that responses are frequently dependent on the cell context or state. This resource article has been published in the journal Cell and all related plasmid reagents are deposited in Addgene. The work in this paper was led by current PhD student Remi Janicot and former postdoc Marcin Maziarz, and includes collaborations with the Layne Lab in the Department of Biochemistry & Cell Biology, and the Wu lab at Stanford.
Congratulations to the Garcia-Marcos Lab!