Appendix O: Boston Public Health Commission Requirements
The Biological Laboratory Regulations adopted by the Boston Public Health Commission (BPHC) as amended in October 2019 requires laboratories working with rDNA and those at Biosafety Level 3 (BSL3) and Biosafety Level 4 (BSL4) to adhere to local standards, including: Obtaining an annual Recombinant DNA permit from the BPHC. An IBC that reports to a […]
Appendix Q: Laboratory and Equipment Decontamination Procedures
Decontamination of Lab Space and Equipment Punch List Assigning a designate a Move Coordinator. The coordinator will work with EHS and other stakeholders to facilitate and coordinate the move. Contact an outside vendor for the decontamination of biological safety cabinets (tissue culture hoods). Have appropriate personal protective equipment available (lab coat, gloves, eye protection). Dispose […]
Appendix S: Laboratory Door Signage
Boston University Door Sign Sample: This is completed in accordance with the NFPA 704 Hazard Identification ratings system (the NFPA “hazard diamond”) for health, flammability, and instability.
Appendix T: Boston University Research Compliance Policy on Disease Surveillance and Reporting for High-Risk Agents
Purpose and Applicability This policy implements BPHC’s Guidelines for Implementation and Enforcement of Boston Public Health Commission’s Disease Surveillance and Reporting Regulation. The BPHC’s guidelines require laboratory registration and a medical surveillance program for research laboratories working with high-risk agents. The guidelines are designed to ensure that BPHC receives timely access to information regarding incidence […]
Appendix U: Criteria for Development of Standard Operating Procedures (SOP)
In this manual, there are a number of sections where the laboratory is required to prepare standard operating procedures (SOPs). This appendix is intended to provide guidelines on the development of such documents. It is not mandatory to follow these procedures. The lab may seek the advice of EHS when developing an SOP. Introduction What […]
Chapter 9: Biohazardous and Medical Waste Disposal
In the Commonwealth of Massachusetts, biohazardous waste is governed by the Department of Public Health regulation 105 CMR 480, “Storage and Disposal of Infectious or Physically Dangerous Medical or Biological Waste, State Sanitary Code Chapter VIII.” Boston University has biological waste management guidance documents for each campus which are reviewed annually and posted on the […]
Chapter 10: Federal Select Agent Program
The U.S. Department of Health and Human Services (DHHS) and the U.S. Department of Agriculture’s (USDA) regulations require institutions that possess, use, or transfer certain biological agents and toxins (known as “select agents”) be registered and approved by the Federal Select Agent Program (FSAP). The FSAP have identified specific biological agents and toxins they consider […]
Chapter 11: Transportation of Biological Materials
Training is required prior to shipping of materials off campus. This does not count as training. The packaging and transportation of biological materials are subject to strict local, state, federal, and international regulations. This is particularly so if the material is transported through the “public domain,” namely, those roadways, airways, and sea lanes accessible to […]
Appendix B: Laboratory Ventilation and Containment for Biosafety
Laboratory-ventilated containment equipment fall into three (3) major categories: Laboratory Chemical (Fume) Hoods Traditional laboratory chemical (or fume) hoods are designed to capture and control chemical vapors and pull them away from the worker. Although the inward flow of air protects the user, chemical hoods do not protect the product (the desired organism being manipulated). […]
Appendix C: Autoclave Quality Assurance Program
Autoclaving is an accepted procedure for the decontamination of certain biohazardous waste. Biological cultures and stocks, contaminated solid waste, and liquid waste can be sterilized through autoclaving. After sterilization in a steam autoclave, these materials are considered non-infectious. All autoclaved waste is placed into the solid biohazard waste stream. Wastes from the BSL4 and BSL3 […]